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This should be a clear focused question that says exactly what you are investigating. It shouldn't be too long and it must include the dependent and independent variables.
Eg. What is the effect of pH on the activity rate of salivary amylase?
Dependent variable: activity rate
Independent variable: pH
This is a paragraph or two where you explain your research question. You are going to say something like:
"Salivary Amylase is a an enzyme that digests starch into di- and monosaccharides. Since it's a salivary amylase, the enzyme works best at an alkaline pH of 7, in other words, the optimum pH is 7. At this pH, the rate of amylase activity will be at it's highest.
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