In addition, the use of the Boyden Chamber assay has great advantages comparing to other motility assays. First, it is versatile in the researching of cell motility, especially in the aspect of intracellular signaling pathways and cell surface proteins on cell motility. Second, the Boyden Chamber assay allows the research of cell motility without the consideration of the interference of cell proliferation and the assay is also time saving. Third, the Boyden Chamber assay can also exclude the interference of the interactions between cells. It’s helpful to research the underlying mechanisms of cell motility.